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OTUs from multiple regions: Greengenes fragment test

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  Greengenes fragment test results
  Making OTUs from different gene regions

Can we make OTUs from different V regions?
This test uses full-length 16S sequences from the Greengenes database. Three different segments are extracted: V3, V4 and V3-V5 using primer pairs commonly used in tag sequencing experiments.

We can now ask if an OTU method assigns different regions of the same full-length sequence to the same cluster. If the method works well, fragments of the same gene should be assigned to the same OTU. See Greengenes fragment test results.