Home Software Services About Contact usearch manual
OTUs from multiple regions: HMP sample validation results

See also
Taxonomy benchmark home
Validating taxonomy classifiers
  Training taxonomy classifiers
  UTAX algorithm
  Splitting a taxonomy reference set
  Defining "accuracy" of a taxonomy classifier
  Taxonomy classification errors

Can we make OTUs from different V regions?
This test uses samples from the Human Microbiome Project that were sequenced using three different primer sets targeting different V regions (V1-V3, V3-V5 and V6-V9). See HMP sample validation for details.

Results are shown in the tables below. The top table shows the weighted Jaccard similarity averaged over samples from each body site. In all cases, the similarity of QIIME closed-ref OTUs are < 10% while the similarity of UTAX OTUs at family and genus level are 67% to 85%.

In the lower table, "hits" indicates the fraction of reads that were classified into OTUs.