Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

New in v11

otutab_binary command

See also
OTU table
OTU commands
Making an OTU table (otutab command)

Convert OTU table from counts to presence (0) / absence (1) values.

Note -- this command is not usually recommended because presence / absence cannot be reliably measured with amplicon sequencing due to cross-talk.

The input file must be in QIIME classic format.

The output file is specified by the -output option.

Example

usearch -otutab_binary otutab.txt -output otutab_bin.txt