Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Sequence database search

  makeudb_sintax  Make UDB database file for sintax
  makeudb_ublast  Make UDB database file for ublast
  makeudb_usearch  Make UDB database file for usearch_global
  search_exact  Search for identical sequences
  search_global  Search database using global alignment without speed heuristics
  search_local  Search database using locbal alignment without speed heuristics
  search_oligodb  Search for matches to short nucleotide sequences, e.g. primers
  search_pcr  In-silico PCR, search for matches to pairs of primers in database
  search_pcr2  In-silico PCR, search for matches to primer pair
  search_peptidedb  Search for matches to short peptide sequences
  search_phix  Search for matches to PhiX sequence
  ublast  Fast database search using local alignment (much faster than BLAST)
  usearch_global  Fast database search using global alignment
  usearch_local  Fast database search using local alignment