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open reading frames

An open reading frame (ORF) is a segment of a nucleotide sequence that begins with a start codon, ends with a stop codon and is long enough to code for a protein. In USEARCH, the minimum number of amino acid codons in an ORF is set by the -mincodons option, default value 20.

With a nucleotide query sequence and amino acid database, USEARCH performs a translated search. ORFs are identified in the nucleotide sequence, and each ORF is treated as a separate query with its own termination conditions. This is because a single nucleotide sequence may span more than one gene.