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SINTAX algorithm

See also
  sintax command
  Microbial taxonomy
  Cross-validation by identity

The SINTAX algorithm predicts the taxonomy of marker gene reads such as 16S or ITS. It is implemented in the sintax command.

Bootstrap confidence values are provided for all predicted ranks.

The algorithm is similar to the RDP Naive Bayesian Classifier algorithm except that k-mer similarity is used to identify the top taxonomy rather than Bayesian posteriors so there is no need for training.

Unlike the RDP Classifier, SINTAX does not require that the lowest ("training") rank be specified for all reference sequences which allows the use of large databases as a reference. However, I do not recommend using SILVA or Greengenes as a taxonomy reference because these databases have high error rates -- roughly one in five of the taxonomy annotations are wrong.